Table 1.
Intra-day accuracy and precision (CV%), n = 5 per Level and inter-day imprecision n = 3
| Analyte | QC level | Intra-day accuracy and precision | Inter-day imprecision | |||
|---|---|---|---|---|---|---|
| Accuracy (%) | CV (%) | Within-day imprecision | Between-day imprecision | Total imprecision | ||
| CV (%) | CV (%) | CV (%) | ||||
| AFA | QC-H | 101.0 ± 4.20 | 4.18 | 5.14 | 0.00a | 5.14 |
| QC-M | 99.9 ± 1.47 | 1.48 | 4.02 | 0.00a | 4.02 | |
| QC-L | 92.9 ± 1.02 | 1.10 | 3.76 | 9.87 | 10.6 | |
| QC-LLOQ | 99.2 ± 2.95 | 2.97 | 4.52 | 4.46 | 6.35 | |
| AXI | QC-H | 93.6 ± 4.75 | 5.07 | 3.30 | 3.67 | 4.94 |
| QC-M | 102.0 ± 5.43 | 5.30 | 4.04 | 0.00a | 4.04 | |
| QC-L | 100.0 ± 3.27 | 3.26 | 2.74 | 0.00a | 2.74 | |
| QC-LLOQ | 99.3 ± 4.77 | 4.81 | 5.41 | 4.12 | 6.80 | |
| BOS | QC-H | 93.3 ± 7.16 | 7.68 | 5.06 | 0.00a | 5.06 |
| QC-M | 94.0 ± 2.52 | 2.68 | 3.41 | 0.52 | 3.45 | |
| QC-L | 90.0 ± 2.81 | 3.13 | 4.75 | 0.00a | 4.75 | |
| QC-LLOQ | 97.8 ± 3.42 | 3.5 | 3.87 | 0.00a | 3.87 | |
| CAB | QC-H | 100.0 ± 2.00 | 1.95 | 5.35 | 1.98 | 5.70 |
| QC-M | 104.0 ± 0.89 | 0.86 | 4.33 | 4.81 | 6.47 | |
| QC-L | 103.0 ± 2.88 | 2.79 | 3.84 | 12.6 | 13.2 | |
| QC-LLOQ | 105.0 ± 3.52 | 3.36 | 4.74 | 12.0 | 12.9 | |
| DAB | QC-H | 102.0 ± 4.73 | 4.65 | 4.11 | 4.64 | 6.20 |
| QC-M | 100.0 ± 3.17 | 3.17 | 3.29 | 3.26 | 4.63 | |
| QC-L | 96.1 ± 5.41 | 5.63 | 4.60 | 7.17 | 8.52 | |
| QC-LLOQ | 102.0 ± 5.71 | 5.59 | 5.23 | 10.4 | 11.6 | |
| LEN | QC-H | 98.6 ± 5.96 | 6.04 | 4.11 | 4.64 | 6.20 |
| QC-M | 99.2 ± 6.55 | 6.60 | 3.29 | 3.26 | 4.63 | |
| QC-L | 99.5 ± 1.21 | 1.21 | 2.73 | 1.35 | 3.04 | |
| QC-LLOQ | 101.0 ± 5.52 | 5.47 | 4.52 | 4.65 | 6.48 | |
| NIL | QC-H | 100.2 ± 5.65 | 5.64 | 3.60 | 2.59 | 4.43 |
| QC-M | 106.0 ± 5.94 | 5.63 | 4.02 | 3.48 | 5.32 | |
| QC-L | 102.0 ± 2.32 | 2.28 | 3.45 | 0.00a | 3.45 | |
| QC-LLOQ | 106.0 ± 5.52 | 5.19 | 4.40 | 2.44 | 5.03 | |
| OSI | QC-H | 94.3 ± 2.43 | 2.57 | 2.57 | 4.38 | 5.08 |
| QC-M | 100.0 ± 3.90 | 3.89 | 3.08 | 6.21 | 6.93 | |
| QC-L | 101.0 ± 4.66 | 4.62 | 3.82 | 8.83 | 9.62 | |
| QC-LLOQ | 99.3 ± 3.93 | 3.96 | 2.83 | 8.30 | 8.77 | |
| RUX | QC-H | 98.1 ± 3.58 | 3.65 | 4.37 | 3.04 | 5.32 |
| QC-M | 102.0 ± 2.82 | 2.76 | 3.19 | 1.73 | 3.63 | |
| QC-L | 93.2 ± 2.45 | 2.63 | 3.23 | 2.17 | 3.89 | |
| QC-LLOQ | 91.7 ± 3.36 | 3.66 | 3.25 | 3.20 | 4.56 | |
| TRA | QC-H | 90.2 ± 7.34 | 8.15 | 2.86 | 0.00a | 2.86 |
| QC-M | 92.3 ± 4.93 | 5.35 | 3.02 | 0.00a | 3.02 | |
| QC-L | 89.03 ± 4.82 | 5.42 | 1.88 | 5.54 | 5.85 | |
| QC-LLOQ | 92.6 ± 5.82 | 6.28 | 7.80 | 5.97 | 9.82 | |
aBetween-day imprecision was set to zero, because variance of day averages was less than weighted variance of pooled within-day imprecision, according to Krouwer and Rabinowitz