Table 3.
HTL epitopes for shortlisted 5 proteins with antigenicity scores.
| Protein | Start amino acid | End amino acid | Peptide | Percentile score | Vaxijen antigenicity | Allergenicity | Toxicity |
|---|---|---|---|---|---|---|---|
| Membrane protein | 16 | 30 | SQIISGIVAAVSAMM | 3.02 | 0.1060 (Probable non-antigen) | Probable allergen | −1.13 (Non-Toxin) |
| 17 | 31 | QIISGIVAAVSAMMW | 3.02 | 0.1324 (Probable non-antigen). | Probable non-allergen | −1.07(Non-Toxin) | |
| 18 | 32 | IISGIVAAVSAMMWI | 4.57 | 0.1871 (Probable non-antigen). | Probable non-allergen | −0.73(Non-Toxin) | |
| Envelope small membrane protein | 44 | 58 | MTGFNTLLVQPALYL | 0.32 | 0.2107 (Probable non-antigen) | Probable non-allergen | −1.52(Non-Toxin) |
| 43 | 57 | CMTGFNTLLVQPALY | 0.71 | 0.1828 (Probable non-antigen). | Probable non-allergen | −1.20(Non-Toxin) | |
| 45 | 59 | TGFNTLLVQPALYLY | 0.88 | 0.1509 (Probable non-antigen) | Probable non-allergen | −1.15(Non-Toxin) | |
| 42 | 56 | QCMTGFNTLLVQPAL | 1.24 | 0.0771 (Probable non-antigen). | Probable non-allergen | −1.28(Non-Toxin) | |
| 46 | 60 | GFNTLLVQPALYLYN | 1.71 | 0.1781 (Probable non-antigen). | Probable non-allergen | −1.26(Non-Toxin) | |
| 27 | 41 | LLVCMAFLTATRLCV | 2.27 | 0.6923 (Probable antigen). | Probable non-allergen | −0.67(Non-Toxin) | |
| 28 | 42 | LVCMAFLTATRLCVQ | 2.51 | 1.0320 (Probable antigen). | Probable non-allergen | −0.56(Non-Toxin) | |
| 26 | 40 | TLLVCMAFLTATRLC | 2.74 | 0.6878 (Probable antigen). | Probable non-allergen | −1.03(Non-Toxin) | |
| 25 | 39 | ITLLVCMAFLTATRL | 2.91 | 0.6608 (Probable antigen). | Probable non-allergen | −1.30(Non-Toxin) | |
| Non-structural protein ORF3 | 6 | 20 | PPTLLLVFSLSLLVT | 0.96 | 0.5922 (Probable antigen). | Probable non-allergen | −1.25 Non-Toxin |
| 7 | 21 | PTLLLVFSLSLLVTA | 0.96 | 0.5642 (Probable antigen). | Probable non-allergen | −1.44(Non-Toxin) | |
| 5 | 19 | RPPTLLLVFSLSLLV | 1.36 | 0.4001 (Probable antigen). | Probable non-allergen | −1.12(Non-Toxin) | |
| 9 | 23 | LLLVFSLSLLVTAFS | 1.58 | 0.5310 (Probable antigen). | Probable non-allergen | −1.36(Non-Toxin) | |
| 4 | 18 | QRPPTLLLVFSLSLL | 1.81 | 0.5021 (Probable antigen). | Probable non-allergen | −1.13(Non-Toxin) | |
| 8 | 22 | TLLLVFSLSLLVTAF | 1.81 | 0.4190 (Probable antigen). | Probable non-allergen | −1.45(Non-Toxin) | |
| 3 | 17 | VQRPPTLLLVFSLSL | 2.05 | 0.3279 (Probable non-antigen). | Probable non-allergen | −1.17(Non-Toxin) | |
| 14 | 28 | SLSLLVTAFSKPLYV | 2.18 | 0.2561 (Probable non-antigen). | Probable non-allergen | −1.48(Non-Toxin) | |
| 13 | 27 | FSLSLLVTAFSKPLY | 4.05 | 0.2759 (Probable non-antigen). | Probable non-allergen | −1.45(Non-Toxin) | |
| Non-structural protein ORF5 | 11 | 25 | LYFLVLYNFLLAIVL | 0.04 | 0.4775 (Probable antigen). | Probable non-allergen | −1.11(Non-Toxin) |
| 10 | 24 | VLYFLVLYNFLLAIV | 0.14 | 0.3188 (Probable non-antigen). | Probable non-allergen | −1.11(Non-Toxin) | |
| 12 | 26 | YFLVLYNFLLAIVLV | 0.14 | 0.5150 (Probable antigen). | Probable non-allergen | −1.32(Non-Toxin) | |
| 13 | 27 | FLVLYNFLLAIVLVN | 0.14 | 0.5732 (Probable antigen). | Probable non-allergen | −1.40(Non-Toxin) | |
| 7 | 21 | RTMVLYFLVLYNFLL | 0.28 | 0.0324 (Probable non-antigen). | Probable allergen | −0.87(Non-Toxin) | |
| 8 | 22 | TMVLYFLVLYNFLLA | 0.28 | 0.2192 (Probable non-antigen). | Probable non-allergen | −0.93(Non-Toxin) | |
| 9 | 23 | MVLYFLVLYNFLLAI | 0.28 | 0.2900 (Probable non-antigen). | Probable non-allergen | −1.18(Non-Toxin) | |
| 35 | 49 | PASGYVAALAVNVCL | 0.77 | 0.0202 (Probable non-antigen). | Probable non-allergen | −0.88(Non-Toxin) | |
| 36 | 50 | ASGYVAALAVNVCLI | 0.77 | 0.1282 (Probable non-antigen). | Probable non-allergen | −0.85(Non-Toxin) | |
| 37 | 51 | SGYVAALAVNVCLIP | 1.06 | 0.3421 (Probable non-antigen). | Probable non-allergen | −0.85(Non-Toxin) | |
| 33 | 47 | YIPASGYVAALAVNV | 1.15 | 0.1243 (Probable non-antigen). | Probable non-allergen | −1.19(Non-Toxin) | |
| 34 | 48 | IPASGYVAALAVNVC | 1.33 | −0.1842 (Probable non-antigen). | Probable allergen | −1.18() | |
| 30 | 44 | DSVPLHIIAPSSLIV | 1.81 | 0.2266 (Probable non-antigen). | Probable non-allergen | −0.90(Non-Toxin) | |
| 28 | 42 | SIVFTYIPASGYVAA | 2.05 | 0.3680 (Probable non-antigen). | Probable non-allergen | −1.12(Non-Toxin) | |
| 29 | 43 | IVFTYIPASGYVAAL | 2.05 | 0.2499 (Probable non-antigen). | Probable non-allergen | −0.84(Non-Toxin) | |
| 27 | 41 | DSIVFTYIPASGYVA | 2.27 | 0.1703 (Probable non-antigen). | Probable allergen | −1.06(Non-Toxin) | |
| 9 | 23 | RSHFIRVSTVSSHGM | 2.51 | 0.1042 (Probable non-antigen). | Probable non-allergen | −1.08(Non-Toxin) | |
| 10 | 24 | SHFIRVSTVSSHGMV | 2.51 | 0.1973 (Probable non-antigen). | Probable non-allergen | −1.11(Non-Toxin) | |
| 8 | 22 | MRSHFIRVSTVSSHG | 2.74 | 0.0550 (Probable non-antigen). | Probable non-allergen | −1.43(Non-Toxin) | |
| 7 | 21 | DMRSHFIRVSTVSSH | 3.36 | 0.3701 (Probable non-antigen). | Probable non-allergen | −1.26(Non-Toxin) | |
| 31 | 45 | FTYIPASGYVAALAV | 3.49 | 0.2843 (Probable non-antigen). | Probable non-allergen | −1.16(Non-Toxin) | |
| 29 | 43 | RDSVPLHIIAPSSLI | 3.88 | 0.2191 (Probable non-antigen). | Probable non-allergen | −1.07(Non-Toxin) | |
| 30 | 44 | VFTYIPASGYVAALA | 3.95 | −0.0681 (Probable non-antigen). | Probable non-allergen | −1.13(Non-Toxin) | |
| 26 | 40 | TDSIVFTYIPASGYV | 4.16 | 0.0533 (Probable non-antigen). | Probable allergen | −0.97(Non-Toxin) | |
| 25 | 39 | STDSIVFTYIPASGY | 4.72 | 0.0749 (Probable non-antigen). | Probable non-allergen | −0.89(Non-Toxin) | |
| Spike protein | 14 | 28 | QSIFYRLNGVGITQQ | 0.39 | 0.7573 (Probable antigen). | Probable non-allergen | −2.15(Non-Toxin) |
| 15 | 29 | SIFYRLNGVGITQQV | 0.62 | 0.7288 (Probable antigen). | Probable non-allergen | −2.30(Non-Toxin) | |
| 4 | 18 | QQLVRSESAALSAQL | 0.62 | 0.3902 (Probable non-antigen). | Probable non-allergen | −1.16(Non-Toxin) | |
| 43 | 57 | IVSFVVNAPNGLYFM | 0.71 | 0.1671 (Probable non-antigen). | Probable non-allergen | −0.39(Non-Toxin) | |
| 38 | 52 | YIWLGFIAGLVALAL | 0.77 | 0.7583 (Probable antigen). | Probable non-allergen | −0.79(Non-Toxin) | |
| 40 | 54 | WLGFIAGLVALALCV | 0.77 | 0.4240 (Probable antigen). | Probable non-allergen | −0.74(Non-Toxin) | |
| 41 | 55 | LGFIAGLVALALCVF | 0.77 | 0.4118 (Probable antigen). | Probable non-allergen | −0.84(Non-Toxin) | |
| 13 | 27 | AQSIFYRLNGVGITQ | 0.79 | 0.6283 (Probable antigen). | Probable non-allergen | −1.97(Non-Toxin) | |
| 46 | 60 | VYDTIKYYSIIPHSI | 0.88 | 0.2157 (Probable non-antigen). | Probable non-allergen | −1.09(Non-Toxin) | |
| 12 | 26 | FAQSIFYRLNGVGIT | 0.96 | 0.4940 (Probable antigen). | Probable non-allergen | −1.77(Non-Toxin) | |
| 39 | 53 | IWLGFIAGLVALALC | 0.96 | 0.4564 (Probable antigen). | Probable non-allergen | −0.77(Non-Toxin) | |
| 16 | 30 | IFYRLNGVGITQQVL | 1.15 | 0.6444 (Probable antigen). | Probable non-allergen | −2.29(Non-Toxin) | |
| 42 | 56 | GFIAGLVALALCVFF | 1.15 | 0.2054 (Probable non-antigen). | Probable allergen | −0.59(Non-Toxin) | |
| 5 | 19 | QLVRSESAALSAQLA | 1.33 | 0.5139 (Probable antigen). | Probable non-allergen | −1.22(Non-Toxin) | |
| 11 | 25 | PFAQSIFYRLNGVGI | 1.36 | 0.4443 (Probable antigen) | Probable non-allergen | −1.66(Non-Toxin) | |
| 44 | 58 | VSFVVNAPNGLYFMH | 1.71 | 0.4899 (Probable antigen). | Probable allergen | −0.43(Non-Toxin) | |
| 3 | 17 | AQQLVRSESAALSAQ | 1.81 | 0.4991 (Probable antigen). | Probable non-allergen | −0.91(Non-Toxin) | |
| 6 | 20 | LVRSESAALSAQLAK | 1.9 | 0.4473 (Probable antigen). | Probable non-allergen | −1.02(Non-Toxin) | |
| 42 | 56 | HIVSFVVNAPNGLYF | 2.18 | 0.1797 (Probable non-antigen). | Probable non-allergen | −0.67(Non-Toxin) | |
| 37 | 51 | WYIWLGFIAGLVALA | 2.27 | 0.6569 (Probable antigen). | Probable non-allergen | −0.72(Non-Toxin) | |
| 17 | 31 | FYRLNGVGITQQVLS | 2.55 | 0.6337 (Probable antigen). | Probable non-allergen | −2.25(Non-Toxin) | |
| 28 | 42 | DFNLTLLEPVSISTG | 2.91 | 1.5583 (Probable antigen). | Probable non-allergen | −1.73(Non-Toxin) | |
| 2 | 16 | VAQQLVRSESAALSA | 3.09 | 0.3358 (Probable non-antigen). | Probable non-allergen | −1.11(Non-Toxin) | |
| 21 | 35 | HGDMYVYSAGHATGT | 3.49 | −0.1383 (Probable non-antigen). | Probable non-allergen | −1.27(Non-Toxin) | |
| 22 | 36 | GDMYVYSAGHATGTT | 3.49 | 0.0241 (Probable non-antigen). | Probable allergen | −0.99(Non-Toxin) | |
| 45 | 59 | SFVVNAPNGLYFMHV | 3.63 | 0.3855 (Probable non-antigen). | Probable non-allergen | −0.48(Non-Toxin) | |
| 23 | 37 | DMYVYSAGHATGTTP | 3.72 | 0.1080 (Probable non-antigen). | Probable non-allergen | −1.03(Non-Toxin) | |
| 9 | 23 | EGGGWLVASGSTVAM | 3.95 | 0.1129 (Probable non-antigen). | Probable non-allergen | −1.03(Non-Toxin) | |
| 10 | 24 | GGGWLVASGSTVAMT | 3.95 | 0.2673 (Probable non-antigen). | Probable non-allergen | −1.12(Non-Toxin) | |
| 43 | 57 | FIAGLVALALCVFFI | 4.16 | 0.2672 (Probable non-antigen). | Probable non-allergen | −0.60(Non-Toxin) | |
| 39 | 53 | NKFNQALGAMQTGFT | 4.77 | 0.3893 (Probable non-antigen). | Probable allergen | −0.79(Non-Toxin) |