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. 2020 Oct 23;11:570872. doi: 10.3389/fimmu.2020.570872

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Copyright © 2020 Su, Zhang, Hu, He, Wang, Xu, Fan, Liu, Zhang and Zhao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NPS2143 blocks the PK2/CaSR pathway and promotes IL-1β secretion. (A) Testicular interstitial fluid was collected from each group, and IL-1β level in the primary TM interstitial fluid was analyzed by ELISA (n= 6/group). (B) LPS, 5% DMSO, 20 mg/kg NPS-2143, MCC950, VX-765, LPS + UPEC + PK2 + DMSO, LPS + UPEC + PK2 + NPS2143, LPS + UPEC + PK2 + MCC950, and LPS + UPEC + PK2 + VX-765 were used to stimulate macrophages for 120 min, and cell supernatants were collected. IL-1β levels in the supernatants of the primary TM were analyzed by ELISA (n=5/group). MCC950 was an inhibitor of NLRP3 protein, and VX-765 was an inhibitor of Caspase-1. (C) Transfection efficiency of PK2 in TM3 cell line (n=3/group). (D) Transfection efficiency of PK2 in RAW 264.7 and J774A.1 (n=3/group). (E–G) The protein level of CaSR was detected by Western blotting. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (t-test).