Fig 4:

Escherichia coli production of eriodictyol and homoeriodictyol. (A) Pathways from tyrosine to the (2S)-flavanone targets naringenin, eriodictyol and homoeriodictyol. New enzyme abbreviations: SeC3H (coumarate 3-hydroxylase from S. espanaensis); PkCOMT (caffeate 3-O-methyltransferase from P. kitakamiensis). (B) Screening a panel of pathways with different 4CL gene candidates for production of eriodictyol from 3 mM caffeic acid substrate, in the presence of cerulenin. (C) Screening a library of ferulic acid pathway constructs for the production of trans-phenylacrylic acids from 3 mM tyrosine substrate. (D) Screening a library of (2S)-flavanone pathway constructs (different 4CL genes) with the best-performing ferulic acid pathway, for production of homoeriodictyol and naringenin (in the presence of cerulenin but without tyrosine). (E) Best-performing constructs for production of homoeriodictyol. Data represent the mean and standard deviation from four replicate cultures (wildtype DH5α cells grown at 30°C for 24 h in TBP media with 0.4% glycerol).