Fig. 4. BCYRN1 inhibits glioma progression by directly binding to miR-619-5p.

a Blast scores of miRNAs aligned to BCYRN1. b The Pearson correlation between lncRNA BCYRN1 level and miR-619-5p level was measured in the same set of glioma tissues (2 Grade I, 9 Grade II, 5 Grade III, 14 Grade IV). The ΔCt values (normalized to GAPDH) were subjected to Pearson correlation analysis (R² = 0.8526, P < 0.0001). c Expression level of miR-619-5p in U251 cells after transfection with overexpression plasmids of BCYRN1. d Expression level of miR-619-5p in T98G cells treated with siRNAs of BCYRN1. e, f RIP was performed using AGO2 antibody in T98G and U251 cell lines transfected with miR-619-5p mimics or mimics NC, then the enrichment of BCYRN1 was detected. g The binding sites of BCYRN1 and miR-619-5p. h Luciferase reporter activity of wild-type (WT) or mutated (MUT) BCYRN1 in 293 T cells co-transfected with miR-619-5p mimics. i MiR-619-5p was pulled down with biotinylated BCYRN1 in T98G and U251 cells; BCYRN1 was pulled down with biotinylated miR-619-5p in T98G and U251 cells. j Colony formation assays in U251 cells transfected with miR-619-5p mimic alone or co-transfection with BCYRN1. k CCK-8 analysis in U251 cells transfected with miR-619-5p mimic alone or co-transfection with BCYRN1. l Flow cytometry in U251 cells transfected with miR-619-5p mimic alone or co-transfection with BCYRN1. m Transwell assays in U251 cells transfected with miR-619-5p mimic alone or co-transfection with BCYRN1. n Wound-healing assays in U251 cells transfected with miR-619-5p mimic alone or co-transfection with BCYRN1. o Relative expression of (j), (l), (m), (n). Error bars, S.E.M. from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****p < 0.0001 by two-tailed Student’s t test. #P was used to show the significance between NC and Si-BCYRN1-2.