Fig. 5. CUEDC2 is a direct target of miR-619-5p.

a Identification of 44 commonly altered targeted mRNAs of miR-619-5p from four public profile datasets (mirDIP, microT-CDS, TargetScan, mirDB). b The Pearson correlation between CUEDC2 level and miR-619-5p level was measured in the same set of glioma tissues (2 Grade I, 9 Grade II, 5 Grade III, 14 Grade IV). The ΔCt values (normalized to GAPDH) were subjected to Pearson correlation analysis (R² = 0.6675, P < 0.0001). c The mRNA and protein expression level of CUEDC2 in T98G cells treated with miR-619-5p mimic or inhibitor. d Luciferase reporter activity of wild-type (WT) or mutated (MUT) CUEDC2 in 293 T cells co-transfected with miR-619-5p mimics. e, f Transwell assays in U251 cells transfected with CUEDC2 alone or co-transfection with miR-619-5p mimics. g CCK-8 analysis in U251 cells transfected with CUEDC2 alone or co-transfection with miR-619-5p mimics. h The Pearson correlation between lncRNA BCYRN1 level and CUEDC2 level was measured in the same set of glioma tissues (2 Grade I, 9 Grade II, 5 Grade III, 14 Grade IV). The ΔCt values (normalized to GAPDH) were subjected to Pearson correlation analysis (R² = 0.4139, P < 0.0001). i, k The mRNA and protein expression level of CUEDC2 in U251 cells treated with transfection of BCYRN1 overexpression plasmid. j, k The mRNA and protein expression level of CUEDC2 in T98G cells treated with BCYRN1 siRNAs. l, n The mRNA and protein expression level of CUEDC2 in T98G cells treated with miR-619-5p mimic alone or co-transfection with BCYRN1. m, n The mRNA and protein expression level of CUEDC2 in U251 cells treated with miR-619-5p inhibitor alone or co-transfection with BCYRN1 siRNAs. Error bars, S.E.M. from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****p < 0.0001 by two-tailed Student’s t test. #P was used to show the significance between NC and Si-BCYRN1-2 or miR-NC and miR-619-5p-inhibitor.