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Inhibition of the AMPK/Sirt3 signaling pathway abolishes mitofusin 2 (Mfn2)-mediated protection. (A) Cell viability was determined through a cell counting kit-8 (CCK8) assay. (B) Lactate dehydrogenase (LDH) release into the medium was measured through ELISA. (C,D) Immunofluorescence assay for mitochondrial reactive oxygen species (mtROS). ∗P < 0.05.
