Table 2.
Histoscore results for the rats from the NC, TI, and EAP groups
| Variable (score values) | NC | TI | EAP |
|---|---|---|---|
| Low power magnification | |||
| Luminal shape regular (1), villous (3), papillary (4), cribriform (5) | 2.72 ± 0.42 | 4.50 ± 0.58* | 3.44 ± 0.44# |
| Acinar shape tubular (1), branched (3), irregular (5) | 2.78 ± 0.79 | 4.72 ± 0.42* | 4.17 ± 0.82* |
| Interacinar space large or moderate (1), back-to-back glands (5) | 2.11 ± 0.99 | 2.83 ± 1.03 | 3.44 ± 1.42* |
| Stroma fine (1), abundant (3), fibrosis/severe SM hyperplasia (5) | 1.22 ± 0.25 | 2.22 ± 0.58* | 3.50 ± 0.53*# |
| High-power magnification | |||
| Epithelial shape flattened (1), cuboidal (1), cylindrical (3), hexagonal (5) | 1.78 ± 0.25 | 4.67 ± 0.41* | 2.56 ± 0.60# |
| Number of layers mono(1); oligo, 2–4 (3); pluri, > 5 (5) | 1.39 ± 0.21 | 4.28 ± 0.79* | 2.22 ± 0.63# |
| If>1, add: focal(3), diffuse(5) | 0.89 ± 0.99 | 3.83 ± 0.94* | 3.44 ± 0.83* |
| Alignment polar (1), apolar (3) | 1.83 ± 0.83 | 2.78 ± 0.25* | 2.78 ± 0.34* |
| If there is piling up of epithelial cells, add 3 | 1.11 ± 0.52 | 2.17 ± 1.18* | 2.72 ± 0.42* |
| If there is budding out of epithelial cells into stroma, add 5 | 0.00 ± 0.00 | 1.83 ± 1.05* | 0.44 ± 0.50# |
| If periacinar clusters of epithelial cells are found, add 3 | 0.33 ± 0.47 | 1.44 ± 0.64* | 2.56 ± 0.93*# |
| If isolated clusters of epithelial cells are found outside acini, add 5 | 0.22 ± 0.42 | 2.00 ± 1.18* | 0.94 ± 0.96# |
| Lesion distribution (for apolar or budding out cells, no lesion = 0): unilobar: isolated (2), multiple (6). bilobar: isolated (4), multiple (8) | 1.72 ± 0.71 | 7.11 ± 1.66* | 6.22 ± 1.99* |
| Nuclear shape round, regular (1); irregular (5) | 1.00 ± 0.00 | 1.83 ± 0.67 | 1.00 ± 0.00 |
| Nuclear size small (2), large (2), small and large in the same acinus (4) | 1.11 ± 0.21 | 2.39 ± 0.46* | 1.33 ± 0.53# |
| Mitoses per field absent (0); isolated, 1–2 (2); abundant, 3–5 (5); excessive, > 5 (10) | 0.00 ± 0.00 | 2.33 ± 0.41* | 0.33 ± 0.62# |
| Basement membrane intact (1); interrupted (5) | 1.17 ± 0.24 | 2.56 ± 0.80* | 1.22 ± 0.42# |
| Thin (1); thick (5) | 1.50 ± 0.41 | 3.83 ± 0.91* | 3.83 ± 0.91* |
| Total score (arbitrary units) | 22.89 ± 1.93 | 57.33 ± 3.47* | 46.17 ± 5.33*# |
Values are expressed as the means ± S.D. *Significant difference compared to the NC group, P < 0.05; #significant difference compared to the TI group, P < 0.05