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. 2020 Oct 23;11:587244. doi: 10.3389/fpls.2020.587244

FIGURE 4.

FIGURE 4

35S:GmNFYA13 Arabidopsis seedlings showed enhanced salt tolerance compared to WT lines. (A) Assessment of salt tolerance in WT and 35S:GmNFYA13 transgenic Arabidopsis plants. Three-week-old seedlings were treated with 350 mM NaCl solution for 7 days. (B–E) The survival rate, ion leakage, MDA, and chlorophyll content of WT and 35S:GmNFYA13 lines were measured after salt treatment. (F) The ABA concentration of WT and 35S:GmNFYA13 lines were measured following salt treatment for 4 days. (G–J) The expression levels of SOS1, SOS2, SOS3, and NCED3 in WT and 35S:GmNFYA13 lines were evaluated under control and salt treatments. Transcript levels of these genes were normalized to those in WT plants under control conditions, and AtTub8 was used as the internal control. Data indicate three biological replicates ± SD. Different letters above the columns represent significant differences at P < 0.05. Each experiment had three biological replicates.