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. 2020 Oct 23;11:587244. doi: 10.3389/fpls.2020.587244

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Copyright © 2020 Ma, Fu, Tang, Yu, Yin, Chen, Zhou, Chen, Xu and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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OE and RNAi transgenic soybean plants, respectively, displayed improved and decreased salt tolerance compared with EV plants. (A) Assessment of salt tolerance in RNAi, EV, and OE transgenic soybean plants. Soybean plants with positive hairy roots were subjected to 500 mM NaCl solution for 7 days. Scale bar = 6 cm. (B) GmNFYA13 transcript was evaluated in RNAi, EV and OE transgenic soybean plants. GmCYP2 was used as the internal control and transcripts of GmNFYA13 were normalized to the expression level in RNAi transgenic soybean plants, which was set as 1.0. Data indicate three biological replicates ± SD. Different letters above the columns represent significant differences at P < 0.05. (C–E) The survival rate, ion leakage, and MDA content in RNAi, EV, and OE transgenic soybean plants. Data indicate three biological replicates (n = 18) ± SD. Different letters above the columns represent significant differences at P < 0.05.