Fig. 4.

TAK-063 treatment does not alter mature astrocytes, microglia, or vessels in post-striatal stroke tissue. a Quantification of GFAP-positive astrocytes showed no differences between TAK-063 treatment groups in mice post-stroke (t value = 0.2513, df = 11). In each panel, a representative tissue stain is shown to the right. b Glut-1 staining in peri-infarct tissue for quantification of vascular endothelial cells post-striatal stroke revealed no differences between treatment groups (t value = 1.461, df = 11). c Quantification of IBA-1 immunoreactivity for microglia/macrophages showed no significant differences in microglia staining in peri-infarct striatal tissue (t value = 1.718, df = 11). Error bars represent mean ± SEM for n = 8–10 per group. Images are taken from a representative stroke + vehicle condition. Scale bar = 50 μm and applies to all photomicrographs. Inset in the top row is a schematic of coronal section with striatal stroke and box indicates location of tissue photomicrogaphs and measurements