Figure 3.

Influence of SYT13 Knockdown on Apoptosis, the Cell Cycle, and Cancer Stemness

(A) Caspase activities (total or individual) were increased by SYT13 knockdown in cells transfected with ASOs. (B) SYT13 knockdown-induced changes in transmembrane mitochondrial potential. The distribution of cells undergoing loss of mitochondrial potential (depolarized/live) was increased by SYT13 knockdown. (C) Cell-cycle analysis. SYT13 knockdown decreased the proportion of cells in G1. (D) Proportions of untreated ALDH-positive cells (control, left) and after ALDH treatment (test, right). (E) Comparison of spheroid formation by MKN1 cells. (F) Forced expression of SYT13 was confirmed using qRT-PCR. Increased proliferation compared with empty plasmid-transfected control cells (MKN74-pCMVentry). ∗p < 0.05. Error bars indicate the standard deviation.