Figure 3.

Radiation-induced damage inhibits the proliferation of intestinal stem cell. (A) ISC proliferation was measured by counting the numbers of pH3-positive cells detected in w¹¹¹⁸ flies irradiated with 100 Gy day 1 and (B) day 14. The result is presented as mean ± SE of at least 10 guts per group. (**p < 0.01, ^nsp > 0.05 by t-test). (C) The effect of irradiation on the ISC number was tested by counting the numbers of Dl-LacZ positive cells. ISC numbers were determined by immunostaining with anti-β-Gal antibody in dissected guts of Dl-LacZ flies on days 1 and (D) day 14 after irradiation. The result is presented as mean ± SE of at least 10 guts per group. (***p < 0.001 by t-test in each group). Error bars indicate SEM (***p < 0.001 by t-test).