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. 2020 Nov 5;11(11):951. doi: 10.1038/s41419-020-03146-w

Fig. 2. GDC-0349 induces NSCLC cell death and apoptosis.

Fig. 2

A549 cells (a–g), the primary human NSCLC cells (NSCLC-1/-2/-3) (j–l), BEAS-2B epithelial cells or the primary human epithelial cells (“Pri-lung epi”) (m and n) were treated with applied concentrations of GDC-0349 (25/100 nM), cells were further cultured for applied time periods, cell death was analyzed and quantified by Trypan blue staining assay (a and j), and caspase activation and apoptosis tested by mentioned assays (b–g, k, l, and n) with cell viability tested by CCK-8 assay (m). A549 cells were pre-treated for 1 h with applied caspase inhibitors (all at 50 μM), followed by GDC-0349 (100 nM) stimulation, cells were further cultured for 48–72 h, when cell viability and apoptosis were examined by CCK-8 (h) and nuclear TUNEL staining (i) assays, respectively. The data are presented as mean ± standard deviation (SD, n = 5). *p < 0.05 vs. “Ctrl” cells. #p < 0.05 vs. GDC-0349 only treatment (h and i). “n.s.” stands for no statistical difference (m and n). The experiments were repeated five times with similar results obtained.