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. Author manuscript; available in PMC: 2021 Feb 28.
Published in final edited form as: Nat Struct Mol Biol. 2020 Aug 31;27(11):1017–1023. doi: 10.1038/s41594-020-0490-9

Extended Data Fig. 7. Tryptophan fluorescence-based RagC XTPase assay.

Extended Data Fig. 7

a, Intrinsic tryptophan fluorescence based RagC XTPase assay of Ragulator-RagAGDP:RagCXTP in the absence (blue) and presence (orange) of SLC38A9NT. Tryptophan fluorescence of a Ragulator-RagAGTP:RagCXTP substrate used in the same assay serves as a positive control (green). Plotted is the mean ± SD mantGDP fluorescence at each time point of one experiment performed in quadruplicates (n=4). The experiment has been performed twice with similar results. norm., normalized. b, Top view of the pre-GAP complex structure illustrating the SLC38A9NT-RagC switch I and GDP (RagA, red) interaction. SLC38A9 (yellow) and RagC switch I (blue) are displayed in surface representation. Data for graph in a is available as source data online.