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. Author manuscript; available in PMC: 2021 Feb 28.
Published in final edited form as: Nat Struct Mol Biol. 2020 Aug 31;27(11):1017–1023. doi: 10.1038/s41594-020-0490-9

Extended Data Fig. 3. HDX-MS analysis of SLC38A9NT in isolation and bound to Rags.

Extended Data Fig. 3

a, Deuterium uptake and peptide coverage (grey lines) of SLC38A9NT in complex with inactive Rags (left) or in isolation (right) at 6, 60, 600 and 60,000 s exchange time. b, HDX difference plots of SLC38A9NT in complex with inactive Rags and in isolation at 60 (left), 600 (middle) and 60,000 s (right) exchange time. Plotted are the mean +/ SD of technical replicates (n=3). c, Individual SLC38A9NT MS peptide spectra of selected peptides in isolation (black) and in complex with inactive Rags (red). Undeuterated reference spectra are shown at the top. Data for graphs in b are available as source data online.