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. 2020 Nov 5;10:19121. doi: 10.1038/s41598-020-76155-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Overview of PARS histologic imaging workflow as compared to conventional light microscopy. (a) Conventional imaging of H&E-stained slides is performed on a bright-field microscope where the Hematoxylin (purple hues) and Eosin (red hues) stains block light from a white source. PARS may image (b) unstained FFPE slide preparations, (c) unstained FFPE blocks and (d) unprocessed tissues by taking advantage of the intrinsic optical absorption provided by the cell nuclei (DNA) and the surrounding cytoplasm (cytochrome). We image each intermediate step along the FFPE process in this paper using a single system configuration to show the versatility of PARS. No other reported technique has reported all of these capabilities in a single modality.