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. 2020 Nov 5;10:19121. doi: 10.1038/s41598-020-76155-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Several comparisons between PARS and conventional bright-field images of FFPE slides of human brain tissues. (a) A wide field of view (WFOV) scan using 266 nm excitation with (b) a matching wide field image of the adjacent slide which has been H&E stained. (c) A two-color (250 nm and 420 nm) PARS with a false-colour map applied to match (d) the adjacent H&E region. Finally (e) and (f) likewise show a two-color PARS and bright-field image respectively in higher detail.