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. 2020 Nov 5;10:19119. doi: 10.1038/s41598-020-75064-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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qPCR analysis confirming transcriptomic results. (A) The 11 genes selected for qPCR analysis showed overall consistency with RNA-seq despite some of the gene expression levels appeared stronger. (B) Correlation between RNA-seq and qPCR gene expression. EF1a is used as a housekeeping gene to calculate relative fold change.