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. Author manuscript; available in PMC: 2021 Mar 9.
Published in final edited form as: Nature. 2020 Sep 9;587(7832):109–114. doi: 10.1038/s41586-020-2714-x

Fig. 2: SUN1:Nesprin-2G complexes control nuclear rotation through dynein interactions.

Fig. 2:

a-c, SUN1 depletion suppresses nuclear rotation. Representative stills from Video 3 and rotational analyses are shown. Western blot is representative of 3 independent experiments. Rotation frequency above or below 180° is shown in c; n = 162 cells total from 3 independent biological replicates, ***p≤0.001. d-e, Effects of Nesprin-2G constructs on nuclear rotation. Representative stills and rotation analyses from Video 4 are in f. Frequency of rotations above or below 180° are in g; bars represent mean ± SEM, n = 127 cells total from 5 independent biological replicates, **p≤0.01, ***p≤0.001, ****p≤0.0001, two-tailed student’s t-test.