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. 2020 Nov 5;10:19087. doi: 10.1038/s41598-020-76100-7

Figure 3.

Figure 3

TatBim-PS induces apoptosis by photoirradiation most efficiently in the G1/S transition phase compared to that in other cell-cycle phases. (A) Scheme of the Fucci-PCI method to investigate cell-cycle-dependent apoptosis. First, HeLa/Fucci2 cells were treated with TatBim-PS for 2 h, and thereafter photoirradiated. Following the irradiation, the cells were incubated and time-lapse images were obtained using the same field of view. The cell cycle phase of each cell was classified using the cellular fluorescence images obtained immediately following the irradiation (left panel). Five hours after photoirradiation, apoptotic cells were stained with NucView405 for 30 min. After the staining, blue NucView405 image (right panel) was captured at the same field as the left and middle panels. (B) Cell-cycle tracking every hour following photoirradiation (TatBim-PS internalization). (C) Light-dependent apoptosis of HeLa/Fucci2 cells at 5.5 h following photoirradiation. Scale bar, 50 µm. (D) Apoptosis rate of cells at each cell cycle phase. Data are shown as the mean ± SEM (n = 3; each of the analyzed areas included 111 cells on average). The p value was determined by one-way ANOVA.