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. 2020 Nov 5;10:19168. doi: 10.1038/s41598-020-76069-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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NGS analysis of the VH and VLK genes in naïve rats. Chord diagram representation of the V and J genes frequency and their respective pairing; analysis of the 5′RACE-amplified cDNA(left) and primer set amplified (right) for VH (a) and VLK (b) genes. The diagrams are color-coded showing the pairing network of the joining regions with each variable gene family, the size of the inner segments correlates with the number of sequences identified for each gene. The frequency of unique CDR3 sequences with different nucleotide lengths in the heavy chain and kappa light chain genes are shown as histograms (c) for both the VH (left) and VLK (right).