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. Author manuscript; available in PMC: 2021 Nov 14.
Published in final edited form as: Chem Commun (Camb). 2020 Oct 15;56(88):13491–13505. doi: 10.1039/d0cc05899h

Fig. 2.

Fig. 2

(a) Fluorescence competition assay to measure the binding affinity of Man-AuNPs with Con A. Man-AuNPs together with Man were incubated with FITC-Con A for 1 h. Fluorescence of the supernatant was measured after removing the bound FITC-Con A by centrifugation. (b) Two equilibria established in the system: Con A with Man-AuNPs and Con A with Man. (c) Cheng-Prusoff equation. IC50: concentration of Man-AuNPs displaying 50% of Man-FITC-Con A binding; [M]: concentration of Man, KD: dissociation constant of Man with Con A, and Kd: dissociation constant of Man-AuNPs with Con A. (d) Fluorescence spectra vs. concentration of Man-AuNPs. (e) Dose response curve of fluorescence vs. concentration of Man-AuNPs. Reproduced from ref. 33 with permission from The American Chemical Society, copyright 2010.