Skip to main content
. Author manuscript; available in PMC: 2021 Nov 14.
Published in final edited form as: Chem Commun (Camb). 2020 Oct 15;56(88):13491–13505. doi: 10.1039/d0cc05899h

Table 1.

Binding affinity of glyco-AuNPs with Con A.33

graphic file with name nihms-1639314-t0011.jpg
Entry Glyco-AuNPs Size (nm)c Na Kd (nM) EFb
1 Man-a-AuNP 22 ± 3.3 107 27.4 ± 2.3 160
2 22 ± 3.3 283 8.67 ± 0.87 192
3 22 ± 3.3 544 1.93 ± 0.25 705
4 22 ± 3.3 1444 1.33 ± 0.31 245
5 22 ± 3.3 2275 1.01 ± 0.22 205
6 22 ± 3.3 3991 0.43 ± 0.044 274
7 Man-b-AuNP 22 ± 3.3 99 92 ± 12 52
8 22 ± 3.3 260 16.1 ± 5.5 112
9 22 ± 3.3 549 12.3 ± 4.1 372
10 22 ± 3.3 1196 10.8 ± 1.8 36
11 22 ± 3.3 2090 7.1 ± 1.7 32
12 22 ± 3.3 2824 4.0 ± 0.7 42
13 Man-a-AuNP 22 ± 3.3 3991 0.43 ± 0.044 274
14 Man-b-AuNP 22 ± 3.3 2824 4.0 ± 0.72 42
15 Man-c-AuNP 22 ± 3.3 1959 15 ± 2.0 16
16 Man-d-AuNP 22 ± 3.3 1590 19 ± 2.2 16
17 Man-b-AuNP 7.2 ± 1.8 297 3.38 ± 0.67 468
18 Man-b-AuNP 14 ± 2.6 1127 3.14 ± 0.49 132
19 Man-b-AuNP 22 ± 3.3 2824 3.99 ± 0.81 42
20 Man-b-AuNP 30 ± 4.0 4486 24.8 ± 3.1 4.2
21 Man-a-AuNP 22 ± 3.3 3,991 0.43 ± 0.044 274
22 Glc-a-AuNP 22 ± 3.3 3,641 12.7 ± 2.5 39
a

N: number of carbohydrate ligands on AuNP, determined by anthrone-sulfuric acid assay

b

Enhancement factor EF = Kd/(KD × N). KD (Man-Con A) = 470 μM; KD (Glc-Con A) = 1.79 mM

c

Diameter of AuNPs was measured by TEM