Table 9.
Binding affinity of Pk-AuNPs and Pk with B-Slt obtained from SPR competition binding assay.64
 | |||||
|---|---|---|---|---|---|
| Entry | Ligand | AuNP size | PK densitya | Kd (M) | EF |
| 1 | PK | -- | -- | 1.14 ×10−4 | -- |
| 2 | PK-s-dimer | -- | -- | 3.68 ×10−5 | -- |
| 3 | PK-l-dimer | -- | -- | 6.38 ×10−6 | -- |
| 4 | 4-PK-s-AuNP | 4 nm | 60 | 1.46 ×10−9 | 420 |
| 5 | 13-PK-s-AuNP | 13 nm | 826 | 8.64 ×10-11 | 516 |
| 6 | 20-PK-s-AuNP | 20 nm | 1,498 | 1.18 ×10−12 | 20,819 |
| 7 | 4-PK-l-AuNP | 4 nm | 113 | 1.95 ×10-11 | 2,895 |
| 8 | 13-PK-l-AuNP | 13 nm | 1,298 | 6.84 ×10−13 | 7,186 |
| 9 | 20-PK-l-AuNP | 20 nm | 1,970 | 2.54 ×10−13 | 12,750 |
a
Determined by the anthrone-sulfuric acid assay.