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. 2020 Nov 5;10:19222. doi: 10.1038/s41598-020-75784-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of OCT-A Capillary Metrics in Normal and NTG eyes. Measurement of OCT-A capillary metrics are generated from ONH OCT-A images by a semi-automated customized MATLAB program (MATLAB R2017a, The MathWorks, Natick, MA, USA. https://www.mathworks.com/products/matlab.html). (From left to right) ONH OCT-A images with the cpVD and cpFD measurement region defined. Binarization of the ONH OCT-A images with white pixels representing the blood vessels and black pixels representing the background (non-perfusion regions). Skeletonization of the ONH OCT-A images. Analysis and quantification of cpVD after removal of retinal arterioles and venules from the binarized ONH OCT-A images. Analysis and quantification of cpFD after removal of retinal arterioles and venules from the skeletonized ONH-OCT-A images. (Top: an example of normal eye. Bottom: an example of NTG eye).