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. 2020 Nov 5;10:19159. doi: 10.1038/s41598-020-76313-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TSCs differentiate to an EVT-like cell type. (A) Morphology representative of EVTs derived from rh121119 TSCs on day 3, 6 and 8 of culture. Scale bar denotes 250 µM. (B) Representative flow cytometry histograms for TSCs, EVTs and rhesus fetal fibroblasts against the following proteins: cytokeratin 7/8, vimentin, Ki-67, Mamu-AG, Mamu-E and CD56. The proportion of cells that are stained (blue) or unstained (red) are represented as the % cells normalized to mode and the x-axis is the fluorescent intensity of the fluorophore. (C) Summary of the flow cytometric analysis represented as the mean and standard deviation (SD) of the median of fluorescence intensity across 7 TSC and EVT lines. A t-test was performed to assess differences in the median of fluorescence intensity between TSCs and EVTs. Control data including stained rhesus fetal fibroblasts and unstained TSCs and EVTs are also shown for comparison. (D) Secretion of MMP2 by EVTs compared to TSCs.