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. 2020 Nov 5;11:5611. doi: 10.1038/s41467-020-19381-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a ci-Ins2 and (b) Ins2 normalized to Hprt1, measured by qPCR, in rat blood cells, plasma, and 19 tissues relative to islet levels. ND = not detected. n = 4 independent experiments. *p < 0.0001 vs. islets by one-way ANOVA and Dunnett post hoc test. c ci-Ins2 (black circles), Ins2 (gray circles), and Gcg (clear circles) normalized to Hprt1, measured by qPCR, in rat α-cells relative to β-cells. n = 5 independent experiments. ***p = 0.000005 (ci-Ins2), ***p = 0.0001 (Ins2), and ***p = 0.0002 (Gcg) vs. β-cells by two-tailed unpaired t-test. a–c Data and mean are represented ± s.d. Source data are provided as a Source Data file.