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. 2020 Nov 5;11:5611. doi: 10.1038/s41467-020-19381-w

Fig. 5. ci-INS is necessary for efficient human β-cell insulin secretion.

Fig. 5

a–f Dissociated human islet cells were transfected with control gapmeR (black circles) or with ci-INS (clear circles) gapmeR 1 (a–c) or gapmeR 2 (d–f). Experiments were performed 48 h after transfection. a, d ci-INS and INS normalized to HPRT1 and PPIA, measured by qPCR, and expressed relative to control in (a) ci-INS gapmeR 1 (n = 6 independent experiments) or (d) ci-INS gapmeR 2 (n = 8 independent experiments). Data and mean are represented ±  s.d. ***p = 0.0001 (a) and ***p = 0.00002 (d) by a two-tailed one-sample t-test. b, e Insulin secretion in response to 2 mM glucose (G), 20 mM G, or 30 mM KCl represented as the fold change vs. control basal (2 mM G) condition in (b) ci-INS gapmeR 1 (n = 6 independent experiments) or (e) ci-INS gapmeR 2 (n = 8 independent experiments). *p = 0.018 (b) and *p = 0.011 (e) by two-tailed paired t-test. c, f Cellular insulin content normalized to protein content in control and (c) ci-INS gapmeR 1 (n = 6 independent experiments) or (f) ci-INS gapmeR 2 (n = 8 independent experiments). Source data are provided as a Source Data file.