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. 2020 Nov 5;11:5611. doi: 10.1038/s41467-020-19381-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a–c Rat islets were transfected with control (black circles) or ci-Ins2 (clear circles) gapmeRs and dissociated into single cells. Experiments were performed 48 h after transfection. a Representative Ca²⁺ imaging in control (black line) or ci-Ins2 (red line) gapmeRs. b Ca²⁺ peak intensity and c integrated Ca²⁺ load after K⁺ stimulation. n = 18 (ci-Ins2 gapmeR) and 19 (control gapmeR) cells from two independent experiments. Data and mean are represented ± s.d. *p = 0.015 and **p = 0.002 by two-tailed unpaired t-test. Source data are provided as a Source Data file.