Fig. 8. ci-Ins2 predominantly localizes to the nucleus and interacts with the RBP TDP-43.

a Expression level of indicated genes, measured by qPCR, in nuclear (clear circles) and cytosolic fractions (black circles) of MIN6B1 cells. n = 4 independent experiments. b RNA immunoprecipitation (IP) of TDP-43 or control IgG in MIN6B1 cell lysates. Left: ci-Ins2 and ciRS-7 RNA levels, measured by qPCR, in immunoprecipitated samples relative to IgG. TDP-43 IP (clear circles) and IgG IP (black circles). n = 4 independent experiments. Right: Representative image of immunoprecipitated samples immunoblotted for TDP-43. Data and mean are represented ± s.d. *p ≤ 0.01 (a) and *p = 0.04 (b) by two-tailed unpaired t-test. Source data are provided as a Source Data file.