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. 2020 Nov 5;6:117. doi: 10.1038/s41420-020-00350-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A, TICs (A) LPMCs (B) were either left unstimulated (Unst) or stimulated with increasing doses of human IL-34 (25–100 ng/ml) for 24 h, and the percentages of live CD45 + , CD68 + ,HLA-DRII + cells expressing CD163 and CD206 were analyzed by flow cytometry. Data are expressed as mean ± SEM of seven experiments. Right panels: representative dotplots showing CD163 and CD206 expression in live CD45 + CD68 + HLA-DRII + TICs (A) or LPMCs (B) isolated from respectively tumoral and non-tumoral samples of one patient with colon rectal cancer (CRC) and treated as described above.