Fig. 2. Rbm8a haploinsufficiency decreases the proliferation of cells in the neocortex and GE.

A Pregnant dams were injected with EdU 1 h prior to dissection of E15 embryos. Embryos were then stained for EdU using click chemistry. Nes-cre; Rbm8a^fl/+ mice, the cortical NPs were significantly decreased in E15 Nes-cre; Rbm8a^fl/+ mice. The number of EdU+ cells were quantified in the neocortex and divided by area (mm²). Scale = 30 µm. *p < 0.05; **p < 0.01; n = 3, Student’s t test. B Nes-cre; Rbm8a^fl/+ mice have significantly fewer cells in the DNA synthesis phase of the cell cycle in the GE. The number of EdU+ cells were quantified in the GE, and divided by the total number of DAPI+ cells and multiplying by 100 to get the percentage of EdU+ cells, *p = 0.01, n = 3, Student’s t test. Scale = 50 µm. C Nes-cre; Rbm8a^fl/+ mice have significantly fewer cells active in the cell cycle (non-quiescent, undifferentiated). E15 brains were stained with cell cycle marker anti-Ki67 antibody. The number of Ki67+ cells were quantified in the GE then divided by the total number of DAPI+ cells and multiplying by 100 to determine the percentage of Ki67+ cells, *p = 0.02, n = 3, Student’s t test. D Western blots showed significant reduction in ARX, FMR1, and FXR2 protein expression in Nes-cre; Rbm8a^fl/+ brain. E. Quantification of protein levels in Western blots of 2D, normalized with ACTIN. *p < 0.05; **p < 0.01; ***p < 0.001; n = 3, Student’s t test.