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. 2020 Nov 5;10:379. doi: 10.1038/s41398-020-01065-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Generation of Nkx2.1-cre; Rbm8a^fl/+; Ai9 mouse model. B Mice have significantly fewer proliferating RFP+ cells in the GE on E17. Scale bar = 50 µm. Quantification of the percentage of proliferating Ki67+ cells normalizing to total RFP+ cells in the GE (***p < 0.001; t test, n = 3). C Analyses of total RFP+ cells in the DLPFC of Nkx2.1-cre; Rbm8a^+/+; Ai9 and Nkx2.1-cre; Rbm8a^fl/+; Ai9 mice. (C1) Representative figures show RFP+ cells in the DLPFC of Nkx2.1-cre; Rbm8a^+/+; Ai9 and Nkx2.1-cre; Rbm8a^fl/+; Ai9 mice. Scale bar = 50 µm. (C2) Quantification of total RFP+ cell density in the DLPFC of Nkx2.1-cre; Rbm8a^+/+; Ai9 and Nkx2.1-cre; Rbm8a^fl/+; Ai9 mice. (C3) Quantification of RFP+ cell density in each bin of the DLPFC. The bar graph shows the RFP+ cell number in each bin divided by area. (C4) Quantification of percentages of RFP+ cells in each bin of the DLPFC. The bar graph shows the percentage of RFP+ cells in each bin to total RFP+ cells in all bins. (C5) Co-immunostaining of PV interneurons in the section shown in C1. Scale bar = 50 µm. (C6) Quantification of total PV+ and RFP+ cell density in the DLPFC. (C7) Quantification of PV+ RFP+ cell density in each bin of the DLPFC. (C8) Quantification of percentages of PV+ RFP+ cells in each bin of the DLPFC. D Analyses of total RFP+ cells in the HP of Nkx2.1-cre; Rbm8a^+/+; Ai9 and Nkx2.1-cre; Rbm8a^fl/+; Ai9 mice. (D1) Representative figures show RFP+ cells in the HP of Nkx2.1-cre; Rbm8a^+/+; Ai9 and Nkx2.1-cre; Rbm8a^fl/+; Ai9 mice. Scale bar = 50 µm. (D2) Quantification of RFP+ cell density in subregions of the HP. The bar graph shows the RFP+ cell number in each subregion divided by area. (D3) Quantification of percentages of RFP+ cells in each subregion of the HP. The bar graph shows the percentage of RFP+ cells in each subregion to total RFP+ cells in the whole HP. (D4) Co-immunostaining of PV interneurons in the section shown in D1. Scale bar = 50 µm. (D5) Quantification of PV+ RFP+ cell density in subregions of the HP. (D6) Quantification of percentages of PV+ RFP+ cells in each subregion of the HP. *p < 0.05; **p < 0.01; ***p < 0.001, t test, n = 3. Bar graphs represent means ± SEMs.