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. 2020 Nov 5;10:19126. doi: 10.1038/s41598-020-75682-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fecundity of adult female mites after treatment with dsRNAs targeting T. urticae orthologs of Tribolium RNAi sensitive genes. dsRNAs were applied at 160 ng/µL. Fecundity is represented as the average number of eggs per female per day laid over 3 days. Significant differences relative to dsRNA-NC were determined by repeated-measures ANOVA followed by pairwise comparisons of treatment estimated marginal means with a Tukey p-value adjustment. (not significant, P > 0.05; *P < 0.05; ***P < 0.001). All experiments were performed in 3 independent trials.