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. 2020 Oct 23;8:557215. doi: 10.3389/fbioe.2020.557215

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Copyright © 2020 Cooke, Ramirez-GarciaLuna, Rangel-Berridi, Park, Nazhat, Weber, Henderson and Rosenzweig.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histological evaluation of subcutaneous implantation of LayFomm scaffold. (A–C) H&E staining of overall tissue morphology; (D–F) α-smooth muscle actin shows formation of fibrous tissue around and directly next to (*) the implanted scaffold; (G–I) CD34 shows positive staining for haematopoietic stem cells, indicating vascularization (arrowheads); (J–L) CD86 staining shows few clusters of M1 macrophages at the scaffold-tissue interface (arrows); (M–O) Arg-1 staining for M2 macrophages is negative.