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. 2020 Oct 23;8:554565. doi: 10.3389/fbioe.2020.554565

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Copyright © 2020 de Melo Pereira, Eischen-Loges, Birgani and Habibovic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Macroscopic image (A) of collagen and biomineralized collagen as used for cell culture experiments; surface structure and elemental composition of collagen (B,D) and biomineralized collagen (C,E) membranes, obtained by SEM-EDS. Low magnifications images of the surface of collagen (B) and biomineralized collagen (C). High magnification image of collagen membrane with corresponding EDS spectrum (D), collagen banding is visible and there is no calcium or phosphorus signal. High magnification image of biomineralized collagen and corresponding EDS spectrum (E), where thicker fibers are visible, and no banding is discernible, expect in one fiber that is not mineralized (yellow arrowheads). Both types of membrane show fibrillar structure.