FIGURE 3.

Diagrammatic rrepresentation of a modified Anderson Cascade Impactor (mACI) with the incorporation of Snapwell or Transwell inserts embedded with respiratory cell lines at the air-liquid interface (ALI). Airflow is maintained at a controlled flow rate and generated via vacuum flow on the opposite end of the mACI to simulate airflow and allow for the assessment of mouth-to-airway transit of drug particles. Evaluation of aerosolized drug particle deposition, transport and absorption across the cell epithelia is determined through assessing inserted Transwells.