Figure 4.

Label-free Quantitative Proteomics between SPOP OE and S119N Mutation

(A) Three repeated protein Venn map showing the SPOP OE sample group by label-free relative quantitative proteomics. (B) Three repeated protein Venn map showing the SPOP S119N sample group by label-free relative quantitative proteomics. (C) Protein Venn map identified between the SPOP OE and SPOP S119N groups. (D) Volcano plot using two factors showing the difference in protein expression between the two sets of samples, and the p value obtained by the t test showing the difference between the two groups of samples. (E) Differentially expressed proteins were found between the SPOP OE and SPOP S119N groups (upregulation more than two times or downregulation less than 0.5, and p value less than 0.05). (F) Heatmap for differentially expressed proteins between SPOP OE and SPOP S119N through label-free relative quantitative proteomics. (G and H) GO (G) and KEGG (H) analyses were performed for differentially expressed proteins between SPOP OE and SPOP S119N through label-free relative quantitative proteomics. (I) Western blot analysis was performed for key proteins of the MAPK signaling pathway.