Figure 5.

Ubiquitinated Label-Free Quantitative Proteomics between SPOP OE and S119N Mutation

(A) Ubiquitinated label-free quantitative proteomics identified 4,327 modified sites, 4,002 modified peptides, and 1,753 modified proteins. (B and C) Three repeated protein Venn maps showing the SPOP OE (B) and SPOP S119N (C) sample groups by ubiquitinated label-free quantitative proteomics. (D) Protein Venn map showing SPOP OE and SPOP S119N groups. (E) Volcano plot showing the differential proteins with ubiquitination of SPOP OE and SPOP S119N groups. (F) Differentially expressed ubiquitination proteins shown between SPOP OE and SPOP S119N groups (upregulation more than two times or downregulation less than 0.5, and p value less than 0.05). (G and H) GO (G) and KEGG (H) analyses were performed for differentially expressed ubiquitination proteins between SPOP OE and SPOP S119N groups.