Figure 8.

Knockdown of SLC7A1 in SPOP S119N Mutation Cells Rescued Cell Proliferation, Migration, and Invasion Phenotype

(A) CCK8 assays showed that SLC7A1 knockdown in SPOP S119N cells inhibited cell proliferation. (B and C) Colony formation assays showed that downregulation of SLC7A1 in SPOP S119N cells inhibited colony formation. (D–F) Transwell assays showed that knockdown of SLC7A1 decreased migration and invasion in SPOP S119N cells. (G) The PI3K/AKT signaling pathway was inhibited by SLC7A1 knockdown in SPOP S119N cells. (H) Relative arginine content was shown in SPOP OE, SPOP S119N, and SLC7A1 knockdown in SPOP S119N cells, respectively. Mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.