Table 1.
Analyses of TEM images from HPF‐FS leaf samples reveal light‐induced changes in thylakoid architecture
| Leaf treatment | Resin curing date | Cryoprotectant | No. of leaf discs (sample blocks) analyzed | Method 1 | Method 2 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample size a (layers of thylakoid repeats) | Lumen + membranes (nm, mean ± SEM) | Stroma gap (nm, mean ± SEM) | Repeat distance (nm, mean ± SEM) | Sample size | Lumen + membranes (nm, mean ± SEM) | Stroma gap (nm, mean ± SEM) | Repeat distance (nm, mean ± SEM) | ||||
| Dark adapted | Jan‐2019 | 300 mM Sorbitol + 10% methanol | 2 | 1,080 | 13.21 ± 0.06 | 3.90 ± 0.03 | 17.09 ± 0.06 | 113 | 12.22 ± 0.21 | 4.68 ± 0.10 | 16.98 ± 0.17 |
| Light adapted | Jan‐2019 | 300 mM Sorbitol + 10% methanol | 2 | 1,080 | 16.33 ± 0.10 | 3.55 ± 0.03 | 19.89 ± 0.10 | 252 | 15.68 ± 0.19 | 4.20 ± 0.07 | 20.04 ± 0.17 |
a
Sample size is the number of Repeat distances analyzed; in general: N(stromal gap) > N(Repeat distance) > N(Lumen + membranes).