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. 2020 Oct 23;11:583378. doi: 10.3389/fmicb.2020.583378

FIGURE 1.

FIGURE 1

Morphological and biological characteristics of vB_BfrS_23. (A) Plaques of vB_BfrS_23D seen on a lawn of B. fragilis GB124. Arrows identify plaques of different sizes (2mm to 0.5mm). (B) Negatively stained TEM images of vB_Bfr_23D. Scale bar = 200nm. (C) One-step growth curve: The latent and rise periods were determined to be approximately 37 and 30 min, respectively, with a rise period and a burst size per cell of approximately 44 phage/cell (mean of 3 replicates). The Average 1 value is the mean PFU/mL of three time points before the rise period with the Average 2 value being the mean PFU/mL of three time point after the one step burst. The error bars depict SEM values (n = 3 biological replicates). The eclipse period was determined to be about 23 min. (D) Temperature assay: The vB_BfrS_23 phage was stable up to 60°C, with declining viability at higher temperatures after 1 h incubation. The error bars depict SEM values (n = 3 biological replicates).