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. 2020 Oct 23;8:577637. doi: 10.3389/fcell.2020.577637

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Copyright © 2020 Azadi, Carmichael, Kovacs, Koster, Kors, Waterham and Schrader.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Correlation of peroxisome morphology and mRNA abundance profiles of PEX genes in HepG2 cells during a time course. (A) Quantitative analysis of tubular peroxisomes in HepG2 cells monitored over time in MEM/FBS and processed for immunofluorescence. Data from 4 independent experiments (n = 300 cells in each condition); analyzed by one-way ANOVA with Dunnett’s post hoc test. (B) qPCR analysis of relative mRNA levels of a set of PEX genes normalized to 18S rRNA expression over time. Note that PEX11β mRNA levels correlate with the proportion of cells showing tubular peroxisome morphology, emphasizing its role in peroxisome elongation and division. In contrast, PEX11α mRNA levels do not correlate with changes in peroxisome morphology. Data from 4 independent experiments; analyzed by one-way ANOVA with Dunnett’s post hoc test; *p < 0.05, **p < 0.01, ***p < 0.001.