Table 3.
Time needed to analyse right and Left-ECC, HCC and SCC.
| Processing steps | |||||||
|---|---|---|---|---|---|---|---|
| Step | SCC (Hours) | Step | Left ECC (hours) | Step | Right ECC (hours) | Step | HCCψ (hours) |
| Centrifugation | 00:20 | N/A | 00:00 | N/A | 00:00 | Technical Time | 0:26 |
| Incubation | 24:00 | ||||||
| Rotating evaporator | 3:00 | ||||||
|
(A) Total Processing Time |
00:20 |
(A) Total Processing Time |
00:00 |
(A) Total Processing Time |
00:00 |
(A) Total Processing Time |
27:26 |
| Analytic Steps | |||||||
| Step |
SCC (hours) |
Step |
Left ECC (hours) |
Step |
Right ECC (hours) |
Step |
HCCψ (hours) |
| Pre-extraction drying of the sample with N2 | N/A | Pre-extraction drying of the sample with N2 | 08:30 | Pre-extraction drying of the sample with N2 | 00:47 | Technical time | 0:06 |
| Extraction of the sample using an organic solvent | N/A | Extraction of the sample using an organic solvent | 02:10 | Extraction of the sample using an organic solvent | 02:10 | Centrifugation | 0:25 |
| Post-extraction drying of the sample with N2 | N/A | Post-extraction drying of the sample with N2 | 00:40 | Post-extraction drying of the sample with N2 | 00:40 | Robot Time | 4:00 |
| Protocol of cortisol extraction | 04:00 | Protocol of cortisol extraction | 04:00 | Protocol of cortisol extraction | 04:00 | ||
| (B) Total analysis Time | 04:00 | (B) Total analysis time | 15:20 | (B) Total analysis time: | 07:37 | (B) Total analysis time | 4:31 |
| Total time: (A + B) | 04:20 | Total time: (A + B) | 15:20 | Total time: (A + B) | 07:37 | Total time: (A + B) | 31:57 |
ψ: These values were obtained thanks to the courtesy of Biomarker Analysis Laboratory quotation at Anglia Ruskin Enterprise (2017), Cambridge; N/A: Non applicable.