Figure 8.

(A) Production of aqueous silk solutions from silk cocoons (1), Fibroin solution (2), Sericin solution (3) (B) Histological image of MI zones of heart for Bone marrow mesenchymal stem cells/silk fibroin/hyaluronic acid (BMSC/SFH) patch shown after 8 weeks of infarction (233). (C) Nanopatterned silk substrate of nanopatterned acid-modified silk fibroin (AMSF) with deposited poly(pyrrole) (PPy) (1 cm²). SEM image of AMSF + PPy nanopatterned substrate. Cardiomyocytes fluorescently stained for α-actinin (green) and nuclei (blue). Cells on nanopatterned substrates exhibit elongated and aligned morphologies. Yellow arrows indicate the direction of the nanopattern. Scale bar: 25 mm; inset 10 mm (234). (D) Genipin crosslinked sericin hydrogel (1) schematic showing the anatomical site (black cross) of the occlusion of left anterior descending coronary artery (LAD) (green line), the corresponding infarcted myocardial region (shaded area), and the injection site of the sericin/genipin hydrogel delivered via a syringe. (2) Macroscopic view of a wild-type heart with a layer of myocardium at the LAD-supplied area cut to open showing an in situ forming of genipin-crosslinked sericin hydrogel (yellow arrowhead). Scale bar, 1 mm (235). (E) Schematic representation of the fabrication of patterned silk films using microgrooved PDMS molds (1). Biocompatibility of silk films with cardiomyocytes: fluorescent microscopy images of confluent monolayers displaying unidirectional alignment of H9c2 (2) and Primary ventricular cardiomyocytes (PCMs) (3) on patterned silk films. Actin cytoskeleton (red: Rhodamine–phalloidin), nucleus [Hoechst 33342 (blue)]. White arrows indicate the direction of the alignment (scale bar−200 mm) (236).