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. 2020 Oct 23;8:588836. doi: 10.3389/fcell.2020.588836

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Copyright © 2020 Lechuga, Naydenov, Feygin, Cruise, Ervasti and Ivanov.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intestinal epithelial-specific knockout of β-actin increases intestinal permeability without causing significant alterations in the structure and composition of epithelial junctions. (A) Intestinal permeability of control and β-actin cKO mice in vivo was determined by measuring gut-to-blood passage of FITC-dextran. Data is presented as a mean ± SE (n = 5); ^∗p < 0.05. (B) Immunoblotting analysis of the expression of selected AJ and TJ proteins in colonic epithelial scrapings obtained from control and β-actin cKO mice. (C) Immunofluorescence labeling and confocal microscopy of AJ (β-catenin and p120 catenin) and TJ (ZO-1, occludin) in colonic sections obtained from control and β-actin cKO mice. Arrows indicate similar localization of junctional proteins in β-actin cKO animals and their control littermates. Scale bar, 20 μm.