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. 2020 Oct 23;8:588836. doi: 10.3389/fcell.2020.588836

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Copyright © 2020 Lechuga, Naydenov, Feygin, Cruise, Ervasti and Ivanov.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intestinal epithelial-specific knockout of β-actin increases colonic expression of proinflammatory cytokines and chemokines during DSS colitis. Control and β-actin cKO mice were exposed to 3% DSS in drinking water, or regular water as a control, for 7 days. mRNA was isolated from the collected colonic samples. Real-time quantitative RT-PCR was used to analyze the expression of different cytokines and chemokines. Data is presented as mean ± SE (n = 5), ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.005.