FIGURE 8.

Loss of intestinal epithelial β-actin increases TNFα-induced cell death ex vivo. Small intestinal crypts were isolated from β-actin cKO and control mice and embedded into Matrigel to generate intestinal enteroids. Differentiated enteroids were treated for 12 h with murine TNFα (100 ng/ml) and the number of dead enteroids, as distinguished by their morphology, was counted. Representative bright field images (A) and quantification of enteroid survival after TNFα treatment (B) are shown. Data is presented as a mean ± SE (n = 3), ^∗p < 0.05. Scale bar, 50 μm.