Figure 3.

Plots Showing the Lipid Abundance in Quality Control Samples from all Seven Plates Both Before and After Batch Correction

(A) Immunofluorescence staining of WT iPSC-differentiated dopamine neurons showing expression of pan-neuronal marker beta-3-tubulin (TUJ1) in red, dopamine marker tyrosine hydroxylase (TH) in green, and nuclear DAPI in blue.

(B) Plot of uncorrected abundance of PC 34:1 in individual QC samples.

(C) Plot of the corrected abundance of PC 34:1, in individual QC samples.

(D) PCA scores plot of quality control samples calculated using all corrected lipid data. Mu, SNCA-A53T mutant dopaminergic neurons; PC, glycerophosphocholine; PCA, principal-component analysis.

(E) PCA scores plot showing the comparability of lipid composition between wild-type cell populations.

(F) PCA scores plot showing the comparability of lipid composition between SNCA-A53T cell populations.